The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab91535-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Expressed by activated B-lymphocytes and monocytes.
References for Anti-CD86 antibody [IT2.2] (PE/Cy5®) (ab95745)
This product has been referenced in:
Kapsogeorgou EK et al. Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4. J Immunol166:3107-13 (2001).
Read more (PubMed: 11207262) »
Jeannin P et al. CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells. J Biol Chem272:15613-9 (1997).
Read more (PubMed: 9188449) »