Overview

  • Product nameAnti-CD89 antibody [EPR4622(2)]
    See all CD89 primary antibodies
  • Description
    Rabbit monoclonal [EPR4622(2)] to CD89
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of Human CD89.

  • Positive control
    • U937, K562, Jurkat, and HL-60 cell lysates; Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4622(2)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

Target

  • FunctionBinds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production.
  • Tissue specificityIsoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD89 antibody
    • Fc alpha receptor antibody
    • Fc fragment of IgA, receptor for antibody
    • FCAR antibody
    • FCAR_HUMAN antibody
    • IgA Fc receptor antibody
    • Immunoglobulin alpha Fc receptor antibody
    see all

Anti-CD89 antibody [EPR4622(2)] images

  • All lanes : Anti-CD89 antibody [EPR4622(2)] (ab124717) at 1/1000 dilution

    Lane 1 : U937 cell lysates
    Lane 2 : K562 cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : HL-60 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 32 kDa
  • ab124717, at 1/100, staining CD89 in paraffin embedded Human tonsil tissue by Immunohistochemistry.

References for Anti-CD89 antibody [EPR4622(2)] (ab124717)

ab124717 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124717.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"