Recombinant
RabMAb

Anti-CD9 antibody [EPR2949] (HRP) (ab195552)

Overview

  • Product name
    Anti-CD9 antibody [EPR2949] (HRP)
    See all CD9 primary antibodies
  • Description
    Rabbit monoclonal [EPR2949] to CD9 (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD9 aa 200 to the C-terminus.

  • Positive control
    • IHC-P: normal human tonsil and normal human kidney tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/71. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.

 

Target

  • Function
    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Tissue specificity
    Expressed by a variety of hematopoietic and epithelial cells.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications
    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

Images

  • IHC image of CD9 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195552 at 1/71 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • IHC image of CD9 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195552 at 1/71 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab195552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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