Anti-CD90 / Thy1 antibody [AF-9] (ab23894)

No signal in IHC on human eye sections.

ANSWER:

Thank you for contacting Abcam earlier today.
I am sorry that you were not getting any staining using ab23894 in IHC-Fr. As we discussed, I have processed a refund, and your credit note number is ********.
Please let me know if there is anything else I can help you with.

                    Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

ANSWER:

Thank you for contacting us.

In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link:

http://www.ncbi.nlm.nih.gov/pubmed/3116408

Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408

Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

The researcher would like to know what secondary was used in ELISA with ab23894. Can we provide a specific protocol?

ANSWER:

I have just received the following information from the laboratory that tested ab23894: "The secondary we used was a home-made goat anti mouse 1:500 in HRPO stabilizer. It is not commercially available. We do not have a specific protocol. Maybe if you can give us more information about what the customer would like to test we will be able to help."

I'm sorry I cannot provide further protocol information in this instance,

I would use this antibody for immunohistochemistry on parrafin sections after ethanol 70? fixation: Is it possible? you only mentionned on frozen sections. Thank you best regards

ANSWER:

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-paraffin sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image).

Please contact us again if you have any additional questions, and happy new year!