Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225)

Dear technical support team:
This customer has highly interested in ab225 (Anti-CD90 / Thy1 antibody [MRC OX-7]), he would likes to order this antibody and test in IHC-Fr with porcine sample, would you please help this customer to create a discount code, the information of this customer as follow:

Thanks for your kindly help.
Best regards

ANSWER:

Thank you for your enquiry and your interest.

Unfortunately, we currently do not have any data (either empirical or from literature) that would support reactivity to porcine tissue. A quick BLAST comparison shows only a 75% homology between the rodent and porcine CD90.

Based on this,we would expect only a weak reactivity to porcine tissue.

flow cytometry with human and rabbit mesenchymal stem cells
problem: no signal
fix: commercial solution
ab11414: Ab dilution not sure
ab225: 1/50
no blocking step
2nd: PE-conj., works well
used controls - also no signal

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab11414andab225(different lots). The order will ship on Monday for delivery on Tuesday.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Has this antibody been tested in pig retina? I would like to know if it specifficaly marks the ganglion cells.

ANSWER:

As far as we are aware, this antibody has not been tested in this application

Has anyone used this antibody in embryonic chick retina - if not can you recommend such an antibody?

ANSWER:

This antibody has not yet been tested in chick so we cannot guarantee that it will work. To date, we no of no others that have been tested in this system.