Anti-CD90 / Thy1 antibody [30-H12] (ab25593)


  • Product nameAnti-CD90 / Thy1 antibody [30-H12]
    See all CD90 / Thy1 primary antibodies
  • Description
    Rat monoclonal [30-H12] to CD90 / Thy1
  • SpecificityMouse CD90.2 (Thy-1.2 alloantigen); Mr 25-30 kDa This antibody does not cross react with strains bearing the Thy 1.1 alloantigen (e.g., AKR/J, PL).
  • Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to CD90/ Thy1. Immunogen is Mouse thymus or spleen.

  • General notes

    This antibody has been shown to be useful in 'in vivo' and 'in vitro' studies of T cell depletion.



Our Abpromise guarantee covers the use of ab25593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-CD90 / Thy1 antibody [30-H12] images

  • ab225593 staining Thy1.2 in murine brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at 4°C. An Alexa Fluor®594-conjugated donkey anti-rat polyclonal was used as secondary antibody at a 1/1000 dilution.

    See Abreview

References for Anti-CD90 / Thy1 antibody [30-H12] (ab25593)

This product has been referenced in:
  • Lopez ME  et al. Neuronal and epithelial cell rescue resolves chronic systemic inflammation in the lipid storage disorder Niemann-Pick C. Hum Mol Genet : (2012). ICC/IF ; Mouse . Read more (PubMed: 22493001) »
  • Koilkonda RD  et al. Induction of rapid and highly efficient expression of the human ND4 complex I subunit in the mouse visual system by self-complementary adeno-associated virus. Arch Ophthalmol 128:876-83 (2010). Read more (PubMed: 20625049) »

See all 4 Publications for this product

Product Wall

I can confirm that we recommend to use between 1ug and 0.3ug per one million cells in flow cytometry.

I am happy to confirm that this clone has been tested and used for decades in flow cytometry and I have added a link to one of the ...

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Application Immunohistochemistry (Frozen sections)
Blocking step NOVOCASTRA protein block as blocking agent as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Sample Mouse Tissue sections (Mouse skeletal muscle)
Specification Mouse skeletal muscle
Permeabilization Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 29 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your reply.

We do not have sample sizes of our products since we carry over 70,000 products and cannot store multiple vial sizes of antibodies in our automated freezers.

We would guarantee ab25593 in IHC-Fr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse brain)
Specification mouse brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 10 2011