Recombinant
RabMAb

Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)

Overview

  • Product name
    Anti-CD90 / Thy1 antibody [EPR3132]
    See all CD90 / Thy1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3132] to CD90 / Thy1
  • Specificity
    This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human CD90/ Thy1 (UniProt ID: P04216).

  • Positive control
    • Fetal brain, fetal kidney lysates, Human breast carcinoma, Human glioma and Human brain tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.

can be blocked with ab134486

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Anti-CD90 / Thy1 antibody [EPR3132] images

    • All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
    • ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab92574 showing positive staining in Normal tonsil vessels tissue.

    • ab92574 showing positive staining in Skeletal muscle vessels tissue.

    • ab92574 showing positive staining in Cervical carcinoma tissue.

    • ab92574 showing positive staining in Lung adenocarcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)

    This product has been referenced in:
    • Sun L  et al. Association between expression of Carboxypeptidase 4 and stem cell markers and their clinical significance in liver cancer development. J Cancer 8:111-116 (2017). Read more (PubMed: 28123604) »
    • Caviedes A  et al. Endothelial Nitric Oxide Synthase Is Present in Dendritic Spines of Neurons in Primary Cultures. Front Cell Neurosci 11:180 (2017). Read more (PubMed: 28725180) »

    See all 20 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Bone marrow biopsy)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Bone marrow biopsy
    Blocking step
    (agent) for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    10% NBF
    Username

    Premier Laboratory

    Verified customer

    Submitted Mar 29 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 13 2014

    Thank you for contacting us.

    The concentration for ab32570 lot GR7483-3 is XXX

    Conc. for ab92574 lot GR37919-4 is XXXX

    Please note these are rabbit monoclonals antibodies. They are very specific to their targets so the workin...

    Read More

    Thank you for contacting us.
    As discussed over the phone, could you please send me the lot numbers?
    Thank you.

    Thank you for contacting us.

    The concentration of the current lot we have in stock (lot GR37919)is 0.0705 mg/mL.

    The blocking peptide has been added to the catalog as ab134486 (http://www.abcam.com/cd90--thy1-peptide-ab134486.html...

    Read More

    Thank you for contacting us.

    I have contacted the lab for the lot concentration as well as for the blocking peptide, and will let you know when I hear back.

    The link to the Human Protein Atlas for human Thy1 is as follows:
    ht...

    Read More

    Thank you for your patience. The lab has confirmed that ab92574 was tested for Flow Cytometry and gave negative results. We do not have any antibodies for CD90 that are known to give good signal in rat but no signal in mouse, and unfortunately I do not...

    Read More

    Thank you for submitting the completed questionnaire and for including the ICC images.

    It seems like the few cells that are stained is just the result of non specific background staining.

    A couple additional questions:

    1. ...

    Read More

    I apologize for the confusion of the datasheet for ab92574. After checking our notes about the non-reactivity with mouse, I found that this antibody does work on mouse samples in WB, but has not worked in mouse samples in other applications. The rat cr...

    Read More

    Thank you for your email. The overall sequence homology for the full length proteins are: human-cow: 74% mouse-cow: 69% rat-cow: 70% The majority of our CD90 antibodies were raised against full length proteins or cell lines and therefore the...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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