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The details of the immunogen for this antibody are not available.
ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.
Our Abpromise guarantee covers the use of ab25272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Cellular Activation||Use at an assay dependent concentration.
In vitro T cells activation: Use at an assay dependent dilution
|Functional Studies||Use at an assay dependent concentration.
Induction of apoptosis: Use at as assay dependent dilution
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.
Use at an assay dependent dilution
Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD90 / Thy1 antibody [G7] (PE/Cy5) ab25272 (1 µg/106cells) and Rat Anti-Mouse CD3-epsilon-PE.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"