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Our Abpromise guarantee covers the use of ab3105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 - 10 µg/ml.|
|IHC-Fr||Use a concentration of 1 - 10 µg/ml. Fix with acetone.|
|IP||Use a concentration of 1 - 10 µg/ml.|
|IHC-FoFr||Use a concentration of 1 - 10 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"