Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

Overview

  • Product nameAnti-CD90 / Thy1 antibody [IBL-6/23]
    See all CD90 / Thy1 primary antibodies
  • Description
    Rat monoclonal [IBL-6/23] to CD90 / Thy1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml. Fix with acetone.
IP Use a concentration of 1 - 10 µg/ml.
IHC-FoFr Use a concentration of 1 - 10 µg/ml.

Target

  • FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-CD90 / Thy1 antibody [IBL-6/23] images

  • ab3105 used in Flow Cytometry.
    ab3105 used at 5µg/ml to stain murine thymus cells. The secondary used was an Alexa Fluor 488 conjugated goat anti-rat IgG monoclonal, used at a 1/1000 dilution.

    See Abreview

  • ab3105 staining CD90 / Thy1 in murine bone marrow cells (smeared directly onto glass slide) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, blocked with 1% serum for 1 hour at 37°C and then incubated with ab3105 at a 1/300 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rat polyclonal used at a 1/400 dilution. Dapi stains nuclei in blue.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.

References for Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

This product has been referenced in:

See 1 Publication for this product

Product Wall

I can confirm that ab3105 was tested on formalin-fixed paraffin-embedded tissue sections from human reactive tonsil but we didn't detect signal with this antibody.

Application Immunohistochemistry (Frozen sections)
Blocking step NOVOCASTRA protein block as blocking agent as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Sample Mouse Tissue sections (Mouse skeletal muscle)
Specification Mouse skeletal muscle
Permeabilization Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Feb 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow cells smear)
Specification Bone marrow cells smear
Fixative Paraformaldehyde
Permeabilization Yes - saponin 0.5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
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Ms. Sophie Nadaud

Verified customer

Submitted Feb 23 2012

Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Thymus)
Specification Thymus
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jul 14 2011

Thank you for your enquiry, we have not tested the antibody ab3105 in immunocytochemistry but the antibody is very likely to work as it works very well in perfusion fixed tissue and frozen post fixed tissue. I would definitely recommend fixing mous...

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