Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225)

Overview

  • Product nameAnti-CD90 / Thy1 antibody [MRC OX-7]
    See all CD90 / Thy1 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-7] to CD90 / Thy1
  • SpecificityRecognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Horse
    Predicted to work with: Mouse, Rabbit, Guinea pig
  • Immunogen

    Full length protein corresponding to Rat CD90/ Thy1.

  • Positive control
    • This antibody gave a positive signal in western blot when tested against rat brain tissue lysate and PC12 whole cell lysate. IF/ICC: PC12 cell line.
  • General notes

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

     

    Alternative versions available:

    Anti-CD90 / Thy1 antibody (HRP) [MRC OX-7] (ab199219)

    Anti-CD90 / Thy1 antibody (FITC) [FITC.MRC OX-7] (ab226)

    Anti-CD90 / Thy1 antibody (Phycoerythrin) [MRC OX-7] (ab33694)

    Anti-CD90 / Thy1 antibody (Biotin) [MRC OX-7] (ab33700)

Properties

Applications

Our Abpromise guarantee covers the use of ab225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23616767
ICC/IF 1/1000.
IHC-Fr Use at an assay dependent concentration. PubMed: 16723538

Target

  • FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-CD90 / Thy1 antibody [MRC OX-7] images

  • ab225 stained PC12 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225) at 5 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 35-37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Rat CD90/Thy1 is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25-37 kDa). 

  • Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feed
  • This image was kindly supplied as part of the review submitted by Nick Voilley. Rat retinal ganglion cell labelled with ab225 as a primary antibody and an anti-mouse F(ab)' 2 coupled to Alexa488 as a secondary antibody. The cell is approximately 15 micrometers in diameter. Only the cells labelled in green in the culture bear action potentials when stimulated. These three elements (reactivity to ab225, size and elecrophysiological parameters) clearly indicate the cell is a ganglion cell.

References for Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225)

This product has been referenced in:
  • Wang F  et al. PTH/SDF-1a cotherapy induces CD90+CD34- stromal cells migration and promotes tissue regeneration in a rat periodontal defect model. Sci Rep 6:30403 (2016). IF ; Rat . Read more (PubMed: 27480134) »
  • Xing S  et al. HDAC is essential for epigenetic regulation of Thy-1 gene expression during LPS/TLR4-mediated proliferation of lung fibroblasts. Lab Invest N/A:N/A (2015). Read more (PubMed: 26214583) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step Other
Sample Rabbit Tissue sections (Bone and cartilage)
Specification Bone and cartilage
Permeabilization No
Fixative 70 % Ethanol
Username

Abcam user community

Verified customer

Submitted Aug 26 2014

Application Flow Cytometry
Sample Mouse Cell (mMSC)
Specification mMSC
Preparation Cell harvesting/tissue preparation method: bone marrow extraction
Sample buffer: PBS
Fixation Methanol
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mMSC)
Loading amount 70 µg
Specification mMSC
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step FBS / BSA in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

Submitted Dec 03 2012

Thank you very much for your call yesterday and for your patience.
I have confirmed with the lab that the antibody is tested with non-permeabilized cells in flow cytometry. So, the epitope is extracellular.
I hope that this information will b...

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Thank you for your enquiry and your interest.

Unfortunately, we currently do not have any data (either empirical or from literature) that would support reactivity to porcine tissue. A quick BLAST comparison shows only a 75% homology between t...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Adipose and bone marrow stem cells)
Specification Adipose and bone marrow stem cells
Fixative Paraformaldehyde
Permeabilization Yes - TRITON x100 0,1%
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Ms. Vega Villar

Verified customer

Submitted Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Application Immunocytochemistry
Sample Rat Cultured Cells (Sciatic nerve Schwann cells and fibroblasts)
Specification Sciatic nerve Schwann cells and fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"