Anti-CD97 antibody - C-terminal (ab155798)
Key features and details
- Rabbit polyclonal to CD97 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD97 antibody - C-terminal
See all CD97 primary antibodies -
Description
Rabbit polyclonal to CD97 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 771-835 of Human CD97 (Uniprot ID P48960)
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Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates, paraffin-embedded Human TOV21G xenograft, methanol-fixed HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 92 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. -
Tissue specificity
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain. -
Domain
The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain. -
Post-translational
modificationsProteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 976 Human
- Omim: 601211 Human
- SwissProt: P48960 Human
- Unigene: 466039 Human
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Alternative names
- ADGRE5 antibody
- Adhesion G protein-coupled receptor E5 antibody
- CD 97 antibody
see all
Images
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All lanes : Anti-CD97 antibody - C-terminal (ab155798) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 92 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human TOV21G xenograft, labeling CD97 with ab155798 at 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells labeling CD97 with ab155798 at 1/200 dilution (green). Bottom image shows costaining with Hoeschst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155798 has not yet been referenced specifically in any publications.