• Product nameAnti-CD97 antibody [EPR4427]
    See all CD97 primary antibodies
  • Description
    Rabbit monoclonal [EPR4427] to CD97
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human CD97.

  • Positive control
    • Human tonsil, Jurkat, U937, and fetal thymus lysates; Human spleen, colonic adenocarcinoma and tonsil tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab108368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/250 - 1/500.
ICC/IF 1/200.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionReceptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
    • Tissue specificityBroadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 5 EGF-like domains.
      Contains 1 GPS domain.
    • DomainThe first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
      Binding to chondroitin sulfate is mediated by the fourth EGF domain.
    • Post-translational
      Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
    • Cellular localizationCell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADGRE5 antibody
      • Adhesion G protein-coupled receptor E5 antibody
      • CD 97 antibody
      • CD_antigen=CD97 antibody
      • CD97 antibody
      • CD97 antigen antibody
      • CD97 antigen subunit beta antibody
      • CD97 molecule antibody
      • CD97_HUMAN antibody
      • Leukocyte antigen CD97 antibody
      • Seven span transmembrane protein antibody
      • Seven transmembrane helix receptor antibody
      • Seven transmembrane heterodimeric receptor associated with inflammation antibody
      • TM7LN 1 antibody
      • TM7LN1 antibody
      see all

    Anti-CD97 antibody [EPR4427] images

    • ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-CD97 antibody [EPR4427] (ab108368) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : U937 cell lysate
      Lane 4 : Human fetal thymus lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 92 kDa
    • ab108368 at 1/250 dilution staining CD97 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108368 at 1/250 dilution staining CD97 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108368 at 1/250 dilution staining CD97 in Human colonic tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-CD97 antibody [EPR4427] (ab108368)

    This product has been referenced in:
    • Safaee M  et al. Overexpression of CD97 confers an invasive phenotype in glioblastoma cells and is associated with decreased survival of glioblastoma patients. PLoS One 8:e62765 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23658650) »

    See 1 Publication for this product

    Product Wall

    J’ai contacté le laboratoire qui m’a confirmé que les IgG de lapins n’ont pas de sous-classes comme les IgG de souris et d'humains. L’isotype d’ab108368 est donc simplement IgG.

    Thank you for contacting us.

    I have spoken to my colleague who suggested ab108368 (http://www.abcam.com/ab108368) to you. This antibody is a rabbit monoclonal which has been tested in immunocytochemistry, immunohistochemistry (paraffin), im...

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    Thank you for your response and for your continued patronage of Abcam. I am glad that you enjoyed the brownies!

    When ordering, it is most beneficial to use our website or to call our customer service representatives as they are much more kno...

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    I has been my pleasure to help with this. I would like to thank you for your help as well and for your cooperation. The details that you have provided will enable us to closely monitor the quality of our products.

    I am sorry this product d...

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    Thank you for sending me this further information. It has helped tremendously in understanding the issues that you have been facing.

    I agree with your assessment that these bands are either non-specific or isoforms 4 and 5. As we cannot be s...

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    I am sorry about the issues you have been having. Thank you for bringing this to our attention. As this product should not be recognizing the other forms of EMR2 it appears that you may be experiencing non specific background issues. I may be able to h...

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    I have had to opportunity to speak with the lab further regarding this. The immunogen sequence for this product is:XXX which spans aaXXX-XXX (SwissProt: Q9UHX3Human). This sequence is within the excellular region and is not in isoforms 4 and 5. Are ...

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    Thank you for contacting us.

    I have had the opportunity to speak with the labs in regard to your questions about these product.

    The immunogen sequence for each product is proprietary but the labs have run alignments and checked ...

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