Overview

  • Product nameAnti-CD97 antibody [MEM-180]
    See all CD97 primary antibodies
  • Description
    Mouse monoclonal [MEM-180] to CD97
  • SpecificityThis antibody recognizes a unique epitope on human CD97 antigen.
  • Tested applicationsSuitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PHA (phytohemagglutinen) activated peripheral blood lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab2527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 - 5 µg/ml.
IP Use at an assay dependent concentration.

Target

  • FunctionReceptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
  • Tissue specificityBroadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 5 EGF-like domains.
    Contains 1 GPS domain.
  • DomainThe first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
    Binding to chondroitin sulfate is mediated by the fourth EGF domain.
  • Post-translational
    modifications
    Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE5 antibody
    • Adhesion G protein-coupled receptor E5 antibody
    • CD 97 antibody
    • CD_antigen=CD97 antibody
    • CD97 antibody
    • CD97 antigen antibody
    • CD97 antigen subunit beta antibody
    • CD97 molecule antibody
    • CD97_HUMAN antibody
    • Leukocyte antigen CD97 antibody
    • Seven span transmembrane protein antibody
    • Seven transmembrane helix receptor antibody
    • Seven transmembrane heterodimeric receptor associated with inflammation antibody
    • TM7LN 1 antibody
    • TM7LN1 antibody
    see all

Anti-CD97 antibody [MEM-180] images

  • Overlay histogram showing U937 cells stained with ab2527 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2527, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CD97 antibody [MEM-180] (ab2527)

This product has been referenced in:

See 1 Publication for this product

Product Wall



We do not measure the extinction coefficient in house, but based on literature, we count with extinction coefficient 1.45.

I have contacted the lab who have confirmed that the epitope for ab2527 has not been localized yet to a particular region of the protein. The only thing known, is that it is somewhere in the extracellular portion of the protein and at different place f...

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