Anti-CD98 antibody [EPR3548(2)] (ab108300)


  • Product name
    Anti-CD98 antibody [EPR3548(2)]
    See all CD98 primary antibodies
  • Description
    Rabbit monoclonal [EPR3548(2)] to CD98
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within the extracellular region of Human CD98

  • Positive control
    • HeLa, HepG2, Raji, and Molt-4 cell lysates, Human brain and Human testis tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.
    • Tissue specificity
      Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
    • Sequence similarities
      Belongs to the SLC3A transporter family.
    • Post-translational
      Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
    • Cellular localization
      Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
    • Information by UniProt
    • Database links
    • Form
      There are 4 isoforms produced by alternative splicing.
    • Alternative names
      • 4F2 antibody
      • 4F2 cell surface antigen heavy chain antibody
      • 4F2 cell-surface antigen heavy chain antibody
      • 4F2 heavy chain antibody
      • 4F2 heavy chain antigen antibody
      • 4F2_HUMAN antibody
      • 4F2hc antibody
      • 4T2HC antibody
      • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
      • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
      • CD 98 antibody
      • CD98 antibody
      • CD98 antigen antibody
      • CD98 heavy chain antibody
      • CD98HC antibody
      • Heavy chain antibody
      • Lymphocyte activation antigen 4F2 large subunit antibody
      • MDU 1 antibody
      • MDU1 antibody
      • Monoclonal antibody 44D7 antibody
      • NACAE antibody
      • Slc3a2 antibody
      • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
      • Solute carrier family 3 member 2 antibody
      see all


    • All lanes : Anti-CD98 antibody [EPR3548(2)] (ab108300) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Molt-4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 68 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108300 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108300 at a dilution of 1/100.


    This product has been referenced in:
    • Robles-Valero J  et al. A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia. Cancer Cell 32:608-623.e9 (2017). Read more (PubMed: 29136506) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting us and sorry for the delay of our reply.

    Please find below the information I obtained for each anti-CD98 antibodies:

    ab2528 : the epitope hasnot beenmapped.
    ab23495 [44D7] reacts with the heavy chain o...

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    Thank you for providing this further information.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to off...

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