Anti-CD99 antibody [12E7] (ab8855)
Key features and details
- Mouse monoclonal [12E7] to CD99
- Suitable for: Flow Cyt, IHC-P, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD99 antibody [12E7]
See all CD99 primary antibodies -
Description
Mouse monoclonal [12E7] to CD99 -
Host species
Mouse -
Specificity
This antibody has been shown to give appropriate reactivity on a wide range of formalin-fixed, paraffin-embedded human tissue sections. -
Tested applications
Suitable for: Flow Cyt, IHC-P, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
12E7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (2) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
Sequence similarities
Belongs to the CD99 family. -
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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Alternative names
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab8855 has been referenced in 6 publications.
- Malleret B et al. Plasmodium vivax binds host CD98hc (SLC3A2) to enter immature red blood cells. Nat Microbiol 6:991-999 (2021). PubMed: 34294905
- Li J et al. Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer. Nat Commun 12:5356 (2021). PubMed: 34531368
- Rooper LM et al. The Utility of NKX2.2 and TLE1 Immunohistochemistry in the Differentiation of Ewing Sarcoma and Synovial Sarcoma. Appl Immunohistochem Mol Morphol 27:174-179 (2019). PubMed: 28800015
- Bode-Lesniewska B et al. EWSR1-NFATC2 and FUS-NFATC2 Gene Fusion-Associated Mesenchymal Tumors: Clinicopathologic Correlation and Literature Review. Sarcoma 2019:9386390 (2019). PubMed: 31049020
- Muff R et al. Explant culture of sarcoma patients' tissue. Lab Invest 96:752-62 (2016). PubMed: 27111283
- Hong SH et al. Pharmacokinetic modeling optimizes inhibition of the 'undruggable' EWS-FLI1 transcription factor in Ewing Sarcoma. Oncotarget 5:338-50 (2014). Human . PubMed: 24481407