• Product nameAnti-CD99 antibody [12E7]
    See all CD99 primary antibodies
  • Description
    Mouse monoclonal [12E7] to CD99
  • SpecificityThis antibody has been shown to give appropriate reactivity on a wide range of formalin-fixed, paraffin-embedded human tissue sections.
  • Tested applicationsSuitable for: IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: Acute lymphocytic leukemia T-cells.



Our Abpromise guarantee covers the use of ab8855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionInvolved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similaritiesBelongs to the CD99 family.
  • Post-translational
    Extensively O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Anti-CD99 antibody [12E7] images

  • ab8855 staining CD99 in the Human Ewing Sarcoma Celline CHP cell line by Flow Cytometry. The sample was incubated with the primary antibody (1/60 in PBS) for 30 minutes at 4°C. A FITC-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
    Gating Strategy: Live Cells.

    See Abreview

References for Anti-CD99 antibody [12E7] (ab8855)

This product has been referenced in:
  • Hong SH  et al. Pharmacokinetic modeling optimizes inhibition of the 'undruggable' EWS-FLI1 transcription factor in Ewing Sarcoma. Oncotarget 5:338-50 (2014). Human . Read more (PubMed: 24481407) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. Yes, this antibody is also clone 12E7.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

The concentration of Lot # GR72758-2 of the anti-CD99 antibody [12E7] is 0.2mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or inf...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Ewing Sarcoma Celline CHP)
Specification Ewing Sarcoma Celline CHP
Fixation No Fixation
Permeabilization No

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Verified customer

Submitted Aug 07 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab8855 in FACS zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für di...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab8855 Anti-CD99 antibody [12E7] bisher noch nicht in FACS getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezie...

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Thank you for contacting Abcam.

We currently do not have any product against the C.elegans protein MKS-5 nor will our products against a similar proteinRPGRIP1L recognize MKS-5. A protein with a similar name is MSK5X (also known as CD99). We...

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Thank you for your enquiry. This antibody was raised against a 24 residue peptide from the MIC2 sequence. Unfortunately, the exact sequence is unknown and epitope mapping has not been carried out.

Thank you for your enquiry. Ab8855 is only available as described on the online datasheet and we are unable to conjugate it to a fluorophore. If you have any more questions, please contact us again.

Yes it contains 0.1% sodium azide.