Overview

  • Product name
  • Description
    Rabbit polyclonal to CD99
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human CD99 protein.

  • Positive control
    • Human pancreas or Ewing's sarcoma for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab27271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: 1/200 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM Citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
    Staining pattern: Cell membrane
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
    • Sequence similarities
      Belongs to the CD99 family.
    • Post-translational
      modifications
      Extensively O-glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12E7 antibody
      • Antigen identified by monoclonal 12E7, Y homolog antibody
      • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
      • CD99 antibody
      • CD99 antigen antibody
      • CD99 molecule antibody
      • CD99_HUMAN antibody
      • Cell surface antigen 12E7 antibody
      • Cell surface antigen HBA 71 antibody
      • Cell surface antigen O13 antibody
      • E2 antigen antibody
      • HBA71 antibody
      • MIC 2X antibody
      • MIC 2Y antibody
      • MIC2 (monoclonal antibody 12E7) antibody
      • MIC2 antibody
      • MIC2X antibody
      • MIC2Y antibody
      • MSK5X antibody
      • Protein MIC2 antibody
      • Surface antigen MIC2 antibody
      • T cell surface glycoprotein E2 antibody
      • T-cell surface glycoprotein E2 antibody
      see all

    Images

    • Ab27271, prediluted, staining CD99 in paraffin embedded, formalin fixed human pancreas tissue by Immunohistochemistry.
    • All lanes : Anti-CD99 antibody (ab27271) at 1 µg/ml

      Lane 1 : Human thymus tissue lysate - total protein (ab30146)
      Lane 2 : Human testis tissue lysate - total protein (ab30257)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 19 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Cooper A  et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res 17:56-66 (2011). IHC-P ; Human . Read more (PubMed: 21047978) »
    • Makhoul R  et al. Paranuclear dot-like immunostaining for CD99: presence in colonic adenomas and adenocarcinomas. Am J Surg Pathol 35:1749-50 (2011). Read more (PubMed: 21997696) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. The immunogen for this antibody is recombinant full length human CD99 protein. This information is available from the online datasheet. I have included a link at the end of this email for your convenience. I hope t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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