Overview

  • Product nameAnti-CD99 antibody [EPR3096]
    See all CD99 primary antibodies
  • Description
    Rabbit monoclonal [EPR3096] to CD99
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the extracellular domains in Human CD99.

  • Positive control
    • THP-1, Jurkat, U937, Molt-4, HuT-78, and HUVEC cell lysates, Human pancreas, Human tonsil and Human uterus tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD99 antibody (Alexa Fluor® 488) [EPR3096] (ab208372)

Properties

Applications

Our Abpromise guarantee covers the use of ab108297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28, 32 kDa (predicted molecular weight: 19 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similaritiesBelongs to the CD99 family.
  • Post-translational
    modifications
    Extensively O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Anti-CD99 antibody [EPR3096] images

  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab108297 at a dilution of 1/250.
  • All lanes : Anti-CD99 antibody [EPR3096] (ab108297) at 1/1000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : Molt-4 cell lysate
    Lane 5 : HuT-78 cell lysate
    Lane 6 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 28,32 kDa (why is the actual band size different from the predicted?)
  •  Flow cytometric analysis of Molt-4 cells using anti-CD99 RabMAb (red) (ab108297) or a rabbit IgG (negative) (green).

  • Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using ab108297. Green-CD99 red-PI

  • Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108297 at a dilution of 1/250.
  • Immunohistochemical staining of paraffin-embedded Human uterus tissue using ab108297 at a dilution of 1/250.

References for Anti-CD99 antibody [EPR3096] (ab108297)

This product has been referenced in:
  • Weekes MP  et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157:1460-72 (2014). WB ; Human . Read more (PubMed: 24906157) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry and your interest in our products.

Each antibody should be titrated to determine the optimal working dilutions. It is the customer's responsibility to calculate how much product is needed for each assay.

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