Anti-CD99 antibody [EPR3097Y] (ab75858)


  • Product nameAnti-CD99 antibody [EPR3097Y]
    See all CD99 primary antibodies
  • Description
    Rabbit monoclonal [EPR3097Y] to CD99
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human CD99

  • Positive control
    • MOLT4, Jurkat and THP1 cell lysates; human Ewing's sarcoma and tonsil tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-CD99 antibody (HRP) [EPR3097Y] (ab194996)



Our Abpromise guarantee covers the use of ab75858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
IP 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionInvolved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
    • Sequence similaritiesBelongs to the CD99 family.
    • Post-translational
      Extensively O-glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12E7 antibody
      • Antigen identified by monoclonal 12E7, Y homolog antibody
      • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
      • CD99 antibody
      • CD99 antigen antibody
      • CD99 molecule antibody
      • CD99_HUMAN antibody
      • Cell surface antigen 12E7 antibody
      • Cell surface antigen HBA 71 antibody
      • Cell surface antigen O13 antibody
      • E2 antigen antibody
      • HBA71 antibody
      • MIC 2X antibody
      • MIC 2Y antibody
      • MIC2 (monoclonal antibody 12E7) antibody
      • MIC2 antibody
      • MIC2X antibody
      • MIC2Y antibody
      • MSK5X antibody
      • Protein MIC2 antibody
      • Surface antigen MIC2 antibody
      • T cell surface glycoprotein E2 antibody
      • T-cell surface glycoprotein E2 antibody
      see all

    Anti-CD99 antibody [EPR3097Y] images

    • ab75858 at 1/1000 dilution staining CD99 in human Ewing's sarcoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab75858 at 1/1000 dilution staining CD99 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-CD99 antibody [EPR3097Y] (ab75858) at 1/5000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    References for Anti-CD99 antibody [EPR3097Y] (ab75858)

    ab75858 has not yet been referenced specifically in any publications.

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