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A synthetic peptide corresponding to residues on the C terminus of human CD99
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab75858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
|Flow Cyt||Use at an assay dependent concentration.|
Flow Cytometry analysis of Jurkat ( Human acute T cell leukemia) labeling CD99 with purified ab75858 at 1/200 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"