Anti-CD99 antibody [EPR3097Y] (ab75858)


  • Product name
    Anti-CD99 antibody [EPR3097Y]
    See all CD99 primary antibodies
  • Description
    Rabbit monoclonal [EPR3097Y] to CD99
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human CD99

  • Positive control
    • MOLT4, Jurkat and THP1 cell lysates; human Ewing's sarcoma and tonsil tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-CD99 antibody (HRP) [EPR3097Y] (ab194996)



Our Abpromise guarantee covers the use of ab75858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
IP 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt Use at an assay dependent concentration.


  • Function
    Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similarities
    Belongs to the CD99 family.
  • Post-translational
    Extensively O-glycosylated.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Anti-CD99 antibody [EPR3097Y] images

  • ab75858 at 1/1000 dilution staining CD99 in human Ewing's sarcoma by Immunohistochemistry, Paraffin-embedded tissue.
  • Flow Cytometry analysis of Jurkat ( Human acute T cell leukemia) labeling CD99 with purified ab75858 at 1/200 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde  and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • ab75858 at 1/1000 dilution staining CD99 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
  • All lanes : Anti-CD99 antibody [EPR3097Y] (ab75858) at 1/5000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 19 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-CD99 antibody [EPR3097Y] (ab75858)

ab75858 has not yet been referenced specifically in any publications.

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We currently do not have any product against the C.elegans protein MKS-5 nor will our products against a similar proteinRPGRIP1L recognize MKS-5. A protein with a similar name is MSK5X (also known as CD99). We...

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