Overview

  • Product nameAnti-CDA antibody
    See all CDA primary antibodies
  • Description
    Rabbit polyclonal to CDA
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide selected from the Center region of human CDA conjugated to KLH.

  • Positive control
    • HeLa cell line and mouse kidney tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesab82347 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
ELISA 1/1000.

Target

  • RelevanceCDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.
  • Database links
  • Alternative names
    • CDD antibody
    • Cytidine aminohydrolase antibody
    • Cytidine deaminase antibody
    • EC 3.5.4.5 antibody

Anti-CDA antibody images

  • ICC/IF image of ab82347 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82347,10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CDA antibody (ab82347) at 1/50 dilution + HeLa cell line lysate at 35 µg

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • Anti-CDA antibody (ab82347) at 1/50 dilution + mouse kidney tissue lysates at 35 µg

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CDA antibody (ab82347)

This product has been referenced in:
  • Kerr M  et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). WB . Read more (PubMed: 25224279) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (multiple tissues)
Loading amount 40 µg
Specification multiple tissues
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"