Overview

  • Product name
    Anti-CDA antibody - C-terminal
    See all CDA primary antibodies
  • Description
    Rabbit polyclonal to CDA - C-terminal
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human CDA aa 118-146 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 978

  • Positive control
    • WB: HeLa cell lysate and mouse kidney tissue lysate. ICC/IF: HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab82346 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • Relevance
    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.
  • Database links
  • Alternative names
    • CDD antibody
    • Cytidine aminohydrolase antibody
    • Cytidine deaminase antibody
    • EC 3.5.4.5 antibody

Images

  • All lanes : Anti-CDA antibody - C-terminal (ab82346) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 16 kDa
  • ab82346 staining CDA in murine pancreatic adenocarcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 5% serum for 1 hour at 25°C and then incubated with ab82346 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an undiluted HRP conjugated horse anti-rabbit polyclonal.

    See Abreview

  • ab82346 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82346 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Damhofer H  et al. Assessment of the stromal contribution to Sonic Hedgehog-dependent pancreatic adenocarcinoma. Mol Oncol N/A:N/A (2013). Read more (PubMed: 23998958) »
  • Frese KK  et al. nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer. Cancer Discov 2:260-269 (2012). Read more (PubMed: 22585996) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung cancer cells)
Loading amount
50000 cells
Specification
lung cancer cells
Treatment
with 10 nM DMSO or paclitaxel for 16 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 27 2012

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification
murine pancreatic adenocarcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

Submitted Jan 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up