The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: Isoform 2, 57 ; Isoform 3, 54 kDa.
FunctionDual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
DomainComposed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1.