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Product Name
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Cdc2 antibody
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See all Cdc2 antibodies (26)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdc2
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Immunogen
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Synthetic peptide (Human) conjugated to KLH.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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No reaction was observed against other related cyclin dependent kinases.
The antibody inhibits the activation of p34cdc2 kinase by cyclins.
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Tested applications
(see key)
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ELISA, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/500 - 1/2000.
IHC-P: 1/200 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Neurology process >> Neural Signal Transduction
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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85.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdc2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Cdc2
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Cdc2 antibody - more information
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