Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdc20
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Cdc20 aa 1-300.
    Sequence:

    MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ PENSQTPTKK EHQKAWALNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWS ASSGDILQLL QMEQPGEYIS SVAWIKEGNY LAVGTSSAEV QLWDVQQQKR LRNMTSHSAR VGSLSWNSYI LSSGSRSGHI HHHDVRVAEH


    Database link: Q12834

  • Positive control
    • NIH 3T3 and INS1 cell lysates; U2OS cells transfected with Cdc20.

Properties

Applications

Our Abpromise guarantee covers the use of ab183479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • Function
    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • Developmental stage
    Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
  • Post-translational
    modifications
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA276H19.3 antibody
    • Cdc 20 antibody
    • CDC20 antibody
    • CDC20 cell division cycle 20 homolog antibody
    • CDC20_HUMAN antibody
    • CDC20A antibody
    • Cell division cycle 20 antibody
    • Cell division cycle 20 homolog (S. cerevisiae) antibody
    • Cell division cycle 20 homolog antibody
    • Cell division cycle protein 20 homolog antibody
    • fizzy antibody
    • MGC102824 antibody
    • p55CDC antibody
    see all

Images

  • All lanes : Anti-Cdc20 antibody (ab183479) at 1/2000 dilution

    Lane 1 : U2OS cells transfected with control si RNA
    Lane 2 : U2OS cells transfected with Cdc20 si RNA
    Lane 3 : Rat INS1 cell lysate
    Lane 4 : Mouse NIH 3T3 cell lysate

    Lysates/proteins at 40 µg per lane.


    Predicted band size : 55 kDa
  • Western blot analysis of Immunoprecipitation using U2OS cells

    Lane 1: Input.

    Lane 2: control serum.

    Lane 3: ab183479.

  • Immunofluorescent analysis of U2OS cells showing labeling of Cdc20 with ab183479 at 1/1000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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