The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
FunctionRequired for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat CDC20/Fizzy family. Contains 7 WD repeats.
Developmental stageSynthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Post-translational modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
Cell division cycle 20 homolog (S. cerevisiae) antibody
Cell division cycle 20 homolog antibody
Cell division cycle protein 20 homolog antibody
Anti-Cdc20 antibody images
Western blot - Cdc20 antibody (ab64877)
All lanes : Anti-Cdc20 antibody (ab64877) at 1/500 dilution
Lane 1 : extract from HeLa cells, (5-30µg total protein). Lane 2 : extract from Jurkat cells, (5-30µg total protein). Lane 3 : extract from Jurkat cells, (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size : 55 kDa Observed band size : 55 kDa Additional bands at : 26 kDa,34 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Cdc20 antibody (ab64877)
This product has been referenced in:
Adhikari D et al. Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. J Cell Biol206:843-53 (2014).
Read more (PubMed: 25246615) »
Uzunova K et al. APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex. Nat Struct Mol Biol19:1116-23 (2012).
Read more (PubMed: 23007861) »