Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdc20
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of human Cdc20 (NP_001246.2).

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the WD repeat CDC20/Fizzy family.
      Contains 7 WD repeats.
    • Developmental stage
      Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
    • Post-translational
      modifications
      Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
      Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA276H19.3 antibody
      • Cdc 20 antibody
      • CDC20 antibody
      • CDC20 cell division cycle 20 homolog antibody
      • CDC20_HUMAN antibody
      • CDC20A antibody
      • Cell division cycle 20 antibody
      • Cell division cycle 20 homolog (S. cerevisiae) antibody
      • Cell division cycle 20 homolog antibody
      • Cell division cycle protein 20 homolog antibody
      • fizzy antibody
      • MGC102824 antibody
      • p55CDC antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cdc20 with ab72202 at 1/1000 (1µg/ml). Detection: DAB.

    References

    ab72202 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab72202.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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