Overview

  • Product nameAnti-Cdc20 antibody
    See all Cdc20 primary antibodies
  • Description
    Rabbit polyclonal to Cdc20
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of Human Cdc20 (NP_001246.2).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues. Mouse teratoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the WD repeat CDC20/Fizzy family.
      Contains 7 WD repeats.
    • Developmental stageSynthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
    • Post-translational
      modifications
      Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
      Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA276H19.3 antibody
      • Cdc 20 antibody
      • CDC20 antibody
      • CDC20 cell division cycle 20 homolog antibody
      • CDC20_HUMAN antibody
      • CDC20A antibody
      • Cell division cycle 20 antibody
      • Cell division cycle 20 homolog (S. cerevisiae) antibody
      • Cell division cycle 20 homolog antibody
      • Cell division cycle protein 20 homolog antibody
      • fizzy antibody
      • MGC102824 antibody
      • p55CDC antibody
      see all

    Anti-Cdc20 antibody images

    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded mouse teratoma tissue by Immunohistochemistry using DAB staining.

    References for Anti-Cdc20 antibody (ab86104)

    ab86104 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86104.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"