Anti-Cdc23 antibody (ab72206)
- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-Cdc23 antibody
See all Cdc23 primary antibodies - DescriptionRabbit polyclonal to Cdc23
- Tested applicationsSuitable for: WB, IPmore details
- Species reactivityReacts with: Human, Xenopus laevis
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Xenopus tropicalis
- Immunogen
Synthetic peptide within Human Cdc23 aa 125-175. The exact sequence is proprietary. (GeneID 8697)
Database link: NP_004652.2 - Positive control
- HeLa cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntibody was affinity purified using an epitope specific to CDC23 immobilized on solid support.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab72206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 68 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
- FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
- PathwayProtein modification; protein ubiquitination.
- Sequence similaritiesBelongs to the APC8/CDC23 family.
Contains 9 TPR repeats. - Post-translational
modificationsPhosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. - Information by UniProt
-
Database links
- Entrez Gene: 8697 Human
- Entrez Gene: 52563 Mouse
- Entrez Gene: 291689 Rat
- Omim: 603462 Human
- SwissProt: Q9UJX2 Human
- SwissProt: Q8BGZ4 Mouse
- Unigene: 73625 Human
- Unigene: 196638 Mouse
see all -
Alternative names
- ANAPC8 antibody
- Anaphase promoting complex subunit 8 antibody
- Anaphase-promoting complex subunit 8 antibody
see all
Anti-Cdc23 antibody images
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Western blot - Cdc23 antibody (ab72206)All lanes : Anti-Cdc23 antibody (ab72206) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size : 68 kDa
References for Anti-Cdc23 antibody (ab72206)
This product has been referenced in:
- Sako K et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014). Xenopus laevis . Read more (PubMed: 24770399) »
- Ohe M et al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). Human, Xenopus laevis . Read more (PubMed: 20089832) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"