Overview

  • Product name
    Anti-Cdc23 antibody
    See all Cdc23 primary antibodies
  • Description
    Rabbit polyclonal to Cdc23
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Cdc23 aa 125-175. The exact sequence is proprietary. (GeneID 8697)
    Database link: NP_004652.2

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC8/CDC23 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ANAPC8 antibody
    • Anaphase promoting complex subunit 8 antibody
    • Anaphase-promoting complex subunit 8 antibody
    • Apc 8 antibody
    • APC8 antibody
    • Cdc 23 antibody
    • CDC23 antibody
    • CDC23_HUMAN antibody
    • cell division cycle 23 antibody
    • Cell division cycle 23 homolog antibody
    • Cell division cycle protein 23 antibody
    • Cell division cycle protein 23 homolog antibody
    • Cut23 antibody
    • Cyclosome subunit 8 antibody
    see all

Images

  • Western blot - Cdc23 antibody (ab72206)
    Western blot - Cdc23 antibody (ab72206)
    All lanes : Anti-Cdc23 antibody (ab72206) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 68 kDa

References

This product has been referenced in:
  • Sako K  et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014). Xenopus laevis . Read more (PubMed: 24770399) »
  • Ohe M  et al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). Human, Xenopus laevis . Read more (PubMed: 20089832) »

See all 2 Publications for this product

Publishing research using ab72206? Please let us know so that we can cite the reference in this datasheet.

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