Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574)


  • Product name
    Anti-Cdc25A (phospho S124) antibody [EPR8888]
    See all Cdc25A primary antibodies
  • Description
    Rabbit monoclonal [EPR8888] to Cdc25A (phospho S124)
  • Host species
  • Specificity
    ab156574 only detects Cdc25A phosphorylated at Serine 124.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 124 of Human Cdc25A (UniProt ID: P30304).

  • Positive control
    • HeLa cell lysate; Human gastric carcinoma and Human testis tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab156574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    • Sequence similarities
      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Domain
      The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
    • Post-translational
      Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc 25a antibody
      • CDC25A antibody
      • CDC25A2 antibody
      • CDC25A2 CAG isoform antibody
      • Cell division cycle 25 homolog A (S. pombe) antibody
      • Cell division cycle 25A antibody
      • Cell division cycle 25A isoform a antibody
      • Cell division cycle 25A isoform b antibody
      • D9Ertd393e antibody
      • Dual specificity phosphatase Cdc25A antibody
      • M phase inducer phosphatase 1 antibody
      • M-phase inducer phosphatase 1 antibody
      • MGC115549 antibody
      • MPIP1_HUMAN antibody
      see all


    • All lanes : Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 59 kDa

    • ab156574, at a 1/1000 dilution, staining Cdc25A (phospho S124) in HeLa lysate immunoprecipitated using ab156574 at 1/10 dilution.
    • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.


    This product has been referenced in:
    • Brill E  et al. Prexasertib, a cell cycle checkpoint kinases 1 and 2 inhibitor, increasesin vitrotoxicity of PARP inhibition by preventing Rad51 foci formation inBRCAwild type high-grade serous ovarian cancer. Oncotarget 8:111026-111040 (2017). Read more (PubMed: 29340034) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Human Cell lysate - whole cell (HeLa)
    10 Gy IR
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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    Verified customer

    Submitted Oct 07 2013


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