Anti-Cdc25A (phospho S178) antibody (ab79252)


  • Product nameAnti-Cdc25A (phospho S178) antibody
    See all Cdc25A primary antibodies
  • Description
    Rabbit polyclonal to Cdc25A (phospho S178)
  • SpecificityDetects Cdc25A only when phosphorylated at serine 178
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human Cdc25A around the phosphorylation site of serine 178 (Q-N-SP-A-P)

  • Positive control
    • WB: extracts from UV-treated A549 cells; IHC-P: Human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab79252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.


  • FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • Sequence similaritiesBelongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • DomainThe phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • Post-translational
    Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 25a antibody
    • CDC25A antibody
    • CDC25A2 antibody
    • CDC25A2 CAG isoform antibody
    • Cell division cycle 25 homolog A (S. pombe) antibody
    • Cell division cycle 25A antibody
    • Cell division cycle 25A isoform a antibody
    • Cell division cycle 25A isoform b antibody
    • D9Ertd393e antibody
    • Dual specificity phosphatase Cdc25A antibody
    • M phase inducer phosphatase 1 antibody
    • M-phase inducer phosphatase 1 antibody
    • MGC115549 antibody
    • MPIP1_HUMAN antibody
    see all

Anti-Cdc25A (phospho S178) antibody images

  • Left panel: immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using 1/50 ab79252. Right panel: same, after pre-incubation with the immunizing peptide.
  • All lanes : Anti-Cdc25A (phospho S178) antibody (ab79252) at 1/500 dilution

    Lane 1 : Extracts from A549 cell treated with UV (15mins)
    Lane 2 : Extracts from A549 cell treated with UV (15mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 110 kDa,117 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Cdc25A (phospho S178) antibody (ab79252)

ab79252 has not yet been referenced specifically in any publications.

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