Overview

  • Product nameAnti-Cdc25B antibody
    See all Cdc25B primary antibodies
  • Description
    Rabbit polyclonal to Cdc25B
  • SpecificityThis antibody detects endogenous levels of total Cdc25B protein.
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human Cdc25B around the phosphorylation site of serine 353.

  • Positive control
    • IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • Sequence similaritiesBelongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Post-translational
    modifications
    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

Anti-Cdc25B antibody images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab59391 at a 1/50 dilution.
    Left image: un-treated.
    Right image: treated with peptide.

References for Anti-Cdc25B antibody (ab59391)

ab59391 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"