• Product name
  • Description
    Rabbit polyclonal to Cdc25B
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the center region of human Cdc25B.

  • Positive control
    • Human breast carcinoma tissue and Ramos cell line lysate.



Our Abpromise guarantee covers the use of ab70927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/50 - 1/100. Detects bands of approximately 65 and 130 kDa (predicted molecular weight: 65 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
    • Sequence similarities
      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Post-translational
      Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI604853 antibody
      • Cdc 25B antibody
      • Cdc25b antibody
      • CDC25HU2 antibody
      • Cdc25m2 antibody
      • Cell division cycle 25 homolog B antibody
      • Cell division cycle 25B antibody
      • Cell division cycle 25B isoform 1 antibody
      • Cell division cycle 25B isoform 2 antibody
      • Cell division cycle 25B isoform 3 antibody
      • Cell division cycle 25B isoform 4 antibody
      • Cell division cycle 25B isoform 5 antibody
      • Dual specificity phosphatase Cdc25B antibody
      • EC antibody
      • M phase inducer phosphatase 2 antibody
      • M-phase inducer phosphatase 2 antibody
      • MPIP2_HUMAN antibody
      see all

    Anti-Cdc25B antibody images

    • Anti-Cdc25B antibody (ab70927) at 1/50 dilution + Ramos cell line lysates at 12.5 µg

      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
    • Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with a70927 at a 1/10 dilution, by Immunohistochemistry. ab70927 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
    • Anti-Cdc25B antibody (ab70927) at 1/100 dilution + Recombinant human Cdc25B protein (ab105906) at 0.01 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Anti-Cdc25B antibody (ab70927)

    This product has been referenced in:
    • Yu XY  et al. MicroRNA-141 is downregulated in human renal cell carcinoma and regulates cell survival by targeting CDC25B. Onco Targets Ther 6:349-54 (2013). WB ; Human . Read more (PubMed: 23596351) »

    See 1 Publication for this product

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