Recombinant
RabMAb

Anti-Cdc25B antibody [EPR3459(2)] (ab124819)

Overview

  • Product name
    Anti-Cdc25B antibody [EPR3459(2)]
    See all Cdc25B primary antibodies
  • Description
    Rabbit monoclonal [EPR3459(2)] to Cdc25B
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cdc25B

  • Positive control
    • K562 cell lysate, U937 cell lysate and THP-1 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
    • Sequence similarities
      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Post-translational
      modifications
      Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI604853 antibody
      • Cdc 25B antibody
      • Cdc25b antibody
      • CDC25HU2 antibody
      • Cdc25m2 antibody
      • Cell division cycle 25 homolog B antibody
      • Cell division cycle 25B antibody
      • Cell division cycle 25B isoform 1 antibody
      • Cell division cycle 25B isoform 2 antibody
      • Cell division cycle 25B isoform 3 antibody
      • Cell division cycle 25B isoform 4 antibody
      • Cell division cycle 25B isoform 5 antibody
      • Dual specificity phosphatase Cdc25B antibody
      • EC 3.1.3.48 antibody
      • M phase inducer phosphatase 2 antibody
      • M-phase inducer phosphatase 2 antibody
      • MPIP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

    References

    ab124819 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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