Anti-Cdc25B antibody [EPR3459(2)] (ab124819)


  • Product name
    Anti-Cdc25B antibody [EPR3459(2)]
    See all Cdc25B primary antibodies
  • Description
    Rabbit monoclonal [EPR3459(2)] to Cdc25B
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cdc25B

  • Positive control
    • K562 cell lysate, U937 cell lysate and THP-1 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
    • Sequence similarities
      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Post-translational
      Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI604853 antibody
      • Cdc 25B antibody
      • Cdc25b antibody
      • CDC25HU2 antibody
      • Cdc25m2 antibody
      • Cell division cycle 25 homolog B antibody
      • Cell division cycle 25B antibody
      • Cell division cycle 25B isoform 1 antibody
      • Cell division cycle 25B isoform 2 antibody
      • Cell division cycle 25B isoform 3 antibody
      • Cell division cycle 25B isoform 4 antibody
      • Cell division cycle 25B isoform 5 antibody
      • Dual specificity phosphatase Cdc25B antibody
      • EC antibody
      • M phase inducer phosphatase 2 antibody
      • M-phase inducer phosphatase 2 antibody
      • MPIP2_HUMAN antibody
      see all

    Anti-Cdc25B antibody [EPR3459(2)] images

    • All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

    References for Anti-Cdc25B antibody [EPR3459(2)] (ab124819)

    ab124819 has not yet been referenced specifically in any publications.

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