Anti-Cdc25B (phospho S149) antibody (ab104739)

Overview

  • Product name
    Anti-Cdc25B (phospho S149) antibody
    See all Cdc25B primary antibodies
  • Description
    Rabbit polyclonal to Cdc25B (phospho S149)
  • Specificity
    ab104739 detects endogenous level Cdc25B protein only when phosphorylated at serine 149.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse Cdc25B aa 147-151 (phospho S149) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (Isoform b).
    Sequence:

    FRS(p)LP


    Database link: NP_001104545.1

  • Positive control
    • 3T3 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • Sequence similarities
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Post-translational
    modifications
    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • Cellular localization
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-Cdc25B (phospho S149) antibody (ab104739) at 1/500 dilution

    Lane 1 : 3T3 cell line lysate (untreated)
    Lane 2 : 3T3 cell line lysate (treated with hydroxyurea)


    Predicted band size : 65 kDa

References

ab104739 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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