Overview

  • Product name
    Anti-Cdc25B (phospho S323) antibody
    See all Cdc25B primary antibodies
  • Description
    Rabbit polyclonal to Cdc25B (phospho S323)
  • Specificity
    Detects endogenous levels of Cdc25B only when phosphorylated at serine 323
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from Human Cdc25B around the phosphorylation site of serine 323 (SPSPMP)

  • Positive control
    • NIH3T3 cell extract treated with PMA

Properties

Applications

Our Abpromise guarantee covers the use of ab53103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/300 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • Sequence similarities
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Post-translational
    modifications
    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • Cellular localization
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-Cdc25B (phospho S323) antibody (ab53103) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins)
    Lane 2 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins) and the immunogenic phosphopeptide.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References

This product has been referenced in:
  • Xu X  et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun 8:14059 (2017). WB ; Human . Read more (PubMed: 28094252) »
  • Zhao X  et al. Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn 244:110-21 (2015). Read more (PubMed: 25349079) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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