Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Sequence similaritiesBelongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Post-translational modificationsPhosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Western blot - Cdc25B (phospho S323) antibody (ab53103)
All lanes : Anti-Cdc25B (phospho S323) antibody (ab53103) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins) Lane 2 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins) and the immunogenic phosphopeptide.
Predicted band size : 65 kDa Observed band size : 65 kDa
References for Anti-Cdc25B (phospho S323) antibody (ab53103)
This product has been referenced in:
Xu X et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun8:14059 (2017).
Read more (PubMed: 28094252) »
Zhao X et al. Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn244:110-21 (2015).
Read more (PubMed: 25349079) »