The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similaritiesBelongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Developmental stageExpressed predominantly in G2 phase.
Post-translational modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
ab84485 at a dilution of 1/250 staining formalin-fixed, paraffin-embedded section of mouse teratoma. Detection: DAB staining.
References for Anti-Cdc25C antibody (ab84485)
This product has been referenced in:
Shirazi Fard S et al. Heterogenic final cell cycle by chicken retinal Lim1 horizontal progenitor cells leads to heteroploid cells with a remaining replicated genome. PLoS One8:e59133 (2013).
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