Overview

  • Product nameAnti-Cdc25C antibody [E303]
    See all Cdc25C primary antibodies
  • Description
    Rabbit monoclonal [E303] to Cdc25C
  • Specificityab32050 recognises Cdc25C. It does not cross react with other MPI phosphatase family members.
  • Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C terminus of human Cdc25C.

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/30.
  • Application notesIs unsuitable for ICC/IF or IHC.
  • Target

    • FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
    • Sequence similaritiesBelongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Developmental stageExpressed predominantly in G2 phase.
    • Post-translational
      modifications
      Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC 25 antibody
      • Cdc 25C antibody
      • CDC25 antibody
      • CDC25C antibody
      • Cell division cycle 25 homolog C antibody
      • Cell division cycle 25C antibody
      • Cell division cycle 25C protein antibody
      • Dual specificity phosphatase Cdc25C antibody
      • M phase inducer phosphatase 3 antibody
      • M-phase inducer phosphatase 3 antibody
      • Mitosis inducer CDC25 antibody
      • MPIP3 antibody
      • MPIP3_HUMAN antibody
      • Phosphotyrosine phosphatase antibody
      • PPP1R60 antibody
      • protein phosphatase 1, regulatory subunit 60 antibody
      see all

    Anti-Cdc25C antibody [E303] images



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human bladder lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32050 was shown to specifically react with Cdc25C when Cdc25C knockout samples were used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab32050 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-Cdc25C antibody [E303] (ab32050) at 1/1000 dilution + A431 cell lysate

      Predicted band size : 53 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    References for Anti-Cdc25C antibody [E303] (ab32050)

    This product has been referenced in:
    • Subramaniam D  et al. Translation inhibition during cell cycle arrest and apoptosis: Mcl-1 is a novel target for RNA binding protein CUGBP2. Am J Physiol Gastrointest Liver Physiol 294:G1025-32 (2008). Read more (PubMed: 18292181) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32050.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"