The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF: Use at a concentration of 2.5 µg/ml.
IP: Use at a concentration of 1 µg/sample.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similaritiesBelongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Developmental stageExpressed predominantly in G2 phase.
Post-translational modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
protein phosphatase 1, regulatory subunit 60 antibody
Anti-Cdc25C antibody [TC-14] images
Western blot - Anti-Cdc25C antibody [TC-14] (ab30313)
Predicted band size : 53 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Hu bladder cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab30313 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa. ab30313 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab30313 and ab181602 (loading control to GAPDH) were diluted at 0.5 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
References for Anti-Cdc25C antibody [TC-14] (ab30313)
has not yet been referenced specifically in any publications.
Publishing research using ab30313? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"