General notesab94219 is a 293T cell transfected lysate in which Human Cdc25C has been transiently over-expressed using a pCMV-Cdc25C plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundDevelopmental stage: Expressed predominantly in G2 phase.
Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
Similarity: Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.