Overview

  • Product name
    Anti-Cdc25C (phospho S216) antibody
    See all Cdc25C primary antibodies
  • Description
    Rabbit polyclonal to Cdc25C (phospho S216)
  • Specificity
    This antibody detects endogenous levels of cdc25C only when phosphorylated at serine 216.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

  • Positive control
    • human breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab47322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ELISA 1/10000.

Target

  • Function
    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage
    Expressed predominantly in G2 phase.
  • Post-translational
    modifications
    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 25 antibody
    • Cdc 25C antibody
    • CDC25 antibody
    • CDC25C antibody
    • Cell division cycle 25 homolog C antibody
    • Cell division cycle 25C antibody
    • Cell division cycle 25C protein antibody
    • Dual specificity phosphatase Cdc25C antibody
    • M phase inducer phosphatase 3 antibody
    • M-phase inducer phosphatase 3 antibody
    • Mitosis inducer CDC25 antibody
    • MPIP3 antibody
    • MPIP3_HUMAN antibody
    • Phosphotyrosine phosphatase antibody
    • PPP1R60 antibody
    • protein phosphatase 1, regulatory subunit 60 antibody
    see all

Images

  • ab47322 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47322 detecting Cdc25C (phospho S216) in Hela Cells (green), together with a secondary goat anti-rabbit antibody conjugated to Cy3®.  Cells were also stained with DAPI in order to highlight the nucleus/ chromosomes (red). Please refer to abreview for further details.

    See Abreview

References

This product has been referenced in:
  • Wang Z  et al. Involvement of ATM-mediated Chk1/2 and JNK kinase signaling activation in HKH40A-induced cell growth inhibition. J Cell Physiol 221:213-20 (2009). WB ; Human . Read more (PubMed: 19530246) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Colon
Blocking step
hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
Username

Vinicius Kannen Cardoso

Verified customer

Submitted Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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