The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/50 - 1/100.
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Expressed predominantly in G2 phase.
Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
ab47322 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
Immunocytochemistry/ Immunofluorescence - Cdc25C (phospho S216) antibody (ab47322)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab47322 detecting Cdc25C (phospho S216) in Hela Cells (green), together with a secondary goat anti-rabbit antibody conjugated to Cy3®. Cells were also stained with DAPI in order to highlight the nucleus/ chromosomes (red). Please refer to abreview for further details.
Wang Z et al. Involvement of ATM-mediated Chk1/2 and JNK kinase signaling activation in HKH40A-induced cell growth inhibition. J Cell Physiol221:213-20 (2009).
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