Anti-Cdc25C (phospho T48) antibody (ab62191)

Overview

  • Product nameAnti-Cdc25C (phospho T48) antibody
    See all Cdc25C primary antibodies
  • Description
    Rabbit polyclonal to Cdc25C (phospho T48)
  • Specificityab62191 detects endogenous levels of CDc25C only when phosphorylated at threonine 48.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human Cdc25C around the phosphorylation site of threonine 48 (P-R-TP-P-V).

  • Positive control
    • Human colon carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similaritiesBelongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stageExpressed predominantly in G2 phase.
  • Post-translational
    modifications
    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 25 antibody
    • Cdc 25C antibody
    • CDC25 antibody
    • CDC25C antibody
    • Cell division cycle 25 homolog C antibody
    • Cell division cycle 25C antibody
    • Cell division cycle 25C protein antibody
    • Dual specificity phosphatase Cdc25C antibody
    • M phase inducer phosphatase 3 antibody
    • M-phase inducer phosphatase 3 antibody
    • Mitosis inducer CDC25 antibody
    • MPIP3 antibody
    • MPIP3_HUMAN antibody
    • Phosphotyrosine phosphatase antibody
    • PPP1R60 antibody
    • protein phosphatase 1, regulatory subunit 60 antibody
    see all

Anti-Cdc25C (phospho T48) antibody images

  • ab62191 at a dilution of 1/50 staining paraffin embedded human colon carcinoma tissue section by immunohistochemistry. Samples were untreated (left hand panel) or treated (right hand panel) with the immunizing peptide.

References for Anti-Cdc25C (phospho T48) antibody (ab62191)

ab62191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"