Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdc27
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    NINDSLEIT

    , corresponding to Internal sequence amino acids 422-430 of Human Cdc27.

  • Positive control
    • Hela whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab21386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/40000.
WB 1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

Images

  • Anti-Cdc27 antibody (ab21386) at 1 µg/ml + Hela whole cell lysate at 35 µg

    Secondary
    Goat anti-Rabbit IgG at 1/10000 dilution

    Predicted band size: 92 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Akimana C  et al. Host factors required for modulation of phagosome biogenesis and proliferation of Francisella tularensis within the cytosol. PLoS One 5:e11025 (2010). WB ; Human . Read more (PubMed: 20552012) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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