The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/40000.
1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
Use at an assay dependent dilution.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein modification; protein ubiquitination.
Belongs to the APC3/CDC27 family. Contains 9 TPR repeats.
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
Akimana C et al. Host factors required for modulation of phagosome biogenesis and proliferation of Francisella tularensis within the cytosol. PLoS One5:e11025 (2010).
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