Overview

  • Product nameAnti-Cdc27 antibody
    See all Cdc27 primary antibodies
  • Description
    Rabbit polyclonal to Cdc27
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 225 and 275 of human Cdc27 (NP_001247.2).

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

Anti-Cdc27 antibody images

  • All lanes : Anti-Cdc27 antibody (ab72214) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Cdc27 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72214 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References for Anti-Cdc27 antibody (ab72214)

This product has been referenced in:
  • Ren YQ  et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015). WB ; Human . Read more (PubMed: 25943633) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"