Overview

  • Product nameAnti-Cdc27 antibody [AF3.1]
    See all Cdc27 primary antibodies
  • Description
    Mouse monoclonal [AF3.1] to Cdc27
  • Tested applicationsSuitable for: IP, Other, WB, IHC-Fr, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.

  • Positive control
    • HeLa cell nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab10538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

Anti-Cdc27 antibody [AF3.1] images

  • All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Mouse myeloid cell lysate


    Predicted band size : 92 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Cdc27 antibody [AF3.1] (ab10538)

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20223764) »
  • Ahlskog JK  et al. Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. Mol Cell Biol 30:5608-20 (2010). WB ; Human . Read more (PubMed: 20937767) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
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Submitted Apr 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"