Overview

  • Product nameAnti-Cdc27 (phospho T244) antibody
    See all Cdc27 primary antibodies
  • Description
    Rabbit polyclonal to Cdc27 (phospho T244)
  • Specificityab12281 is specific for the phosphorylated form of the Cdc27 (phospho T244). Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. The antibody does not cross-react with Cdc27 phosphorylated at other sites.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide: C-VISPDTpVPLGT conjugated to KLH, corresponding to amino acids 240-248 of Human cdc27.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC/IF
  • Application notesELISA: 1/3000 - 1/15000. Reactivity with non-phosphorylated human Cdc27 is minimal by ELISA.
    IF: Use at an assay dependent dilution.
    WB: 1/300 - 1/2000. Detects a band of approximately 92 kDa.
    ICC/IF: Use at an assay dependent dilution (from PubMed: 17472438).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the APC3/CDC27 family.
      Contains 9 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC3 antibody
      • Anaphase Promoting Complex 3 antibody
      • Anaphase promoting complex protein 3 antibody
      • Anaphase Promoting Complex Subunit 3 antibody
      • Anaphase-promoting complex subunit 3 antibody
      • APC 3 antibody
      • APC3 antibody
      • Cdc 27 antibody
      • Cdc27 antibody
      • CDC27 homolog antibody
      • CDC27_HUMAN antibody
      • CDC27Hs antibody
      • Cell Division Cycle 27 antibody
      • Cell division cycle protein 27 homolog antibody
      • D0S1430E antibody
      • D17S978E antibody
      • H NUC antibody
      • H-NUC antibody
      • HNUC antibody
      • Nuc2 homolog antibody
      see all

    Anti-Cdc27 (phospho T244) antibody images

    • All lanes : Anti-Cdc27 (phospho T244) antibody (ab12281) at 1/500 dilution

      Lane 1 : HeLa whole cell lysate from asynchronous cells
      Lane 2 : HeLa whole cell lysate from cells treated with nocodazole

      Lysates/proteins at 30 µg per lane.

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG [H&L] MX at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 92 kDa (why is the actual band size different from the predicted?)

    References for Anti-Cdc27 (phospho T244) antibody (ab12281)

    This product has been referenced in:
    • Skoufias DA  et al. Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed. J Cell Biol 179:671-85 (2007). WB ; Human . Read more (PubMed: 18025303) »
    • Lindqvist A  et al. Cyclin B1-Cdk1 activation continues after centrosome separation to control mitotic progression. PLoS Biol 5:e123 (2007). ICC/IF ; Human . Read more (PubMed: 17472438) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab12281.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"