Overview

  • Product nameAnti-CDC2L5 antibody
    See all CDC2L5 primary antibodies
  • Description
    Mouse monoclonal to CDC2L5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CDC2L5 aa 615-926.

Properties

Applications

Our Abpromise guarantee covers the use of ab58309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis. In case of infection by HIV-1 virus, interacts with HIV-1 Tat protein acetylated at 'Lys-50' and 'Lys-51', thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.
    • Tissue specificityExpressed in fetal brain, liver, muscle and in adult brain. Also expressed in neuroblastoma and glioblastoma tumors.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus speckle.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC2 related protein kinase 5 antibody
      • CDC2-related protein kinase 5 antibody
      • CDC2L antibody
      • CDK13 antibody
      • CDK13_HUMAN antibody
      • cell division controller cholinesterase related antibody
      • cell division cycle 2 like 5 (cholinesterase related cell division controller) antibody
      • cell division cycle 2 like 5 antibody
      • Cell division cycle 2 like protein kinase 5 antibody
      • Cell division cycle 2-like protein kinase 5 antibody
      • Cell division protein kinase 13 antibody
      • CHED antibody
      • Cholinesterase related cell division controller antibody
      • Cholinesterase-related cell division controller antibody
      • cyclin dependent kinase 13 antibody
      • Cyclin-dependent kinase 13 antibody
      • hCDK13 antibody
      see all

    Anti-CDC2L5 antibody images

    • CDC2L5 antibody (ab58309) used in immunofluorescence at 10ug/ml on HeLa cells.


    • Predicted band size : 165 kDa
      CDC2L5 antibody (ab58309) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-CDC2L5 antibody (ab58309)

    ab58309 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Treatment siRNA 48hrs
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Nov 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"