Overview

  • Product name
  • Description
    Mouse monoclonal to CDC2L5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CDC2L5 aa 615-926.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab58309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis. In case of infection by HIV-1 virus, interacts with HIV-1 Tat protein acetylated at 'Lys-50' and 'Lys-51', thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.
    • Tissue specificity
      Expressed in fetal brain, liver, muscle and in adult brain. Also expressed in neuroblastoma and glioblastoma tumors.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus speckle.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC2 related protein kinase 5 antibody
      • CDC2-related protein kinase 5 antibody
      • CDC2L antibody
      • CDK13 antibody
      • CDK13_HUMAN antibody
      • cell division controller cholinesterase related antibody
      • cell division cycle 2 like 5 (cholinesterase related cell division controller) antibody
      • cell division cycle 2 like 5 antibody
      • Cell division cycle 2 like protein kinase 5 antibody
      • Cell division cycle 2-like protein kinase 5 antibody
      • Cell division protein kinase 13 antibody
      • CHED antibody
      • Cholinesterase related cell division controller antibody
      • Cholinesterase-related cell division controller antibody
      • cyclin dependent kinase 13 antibody
      • Cyclin-dependent kinase 13 antibody
      • hCDK13 antibody
      see all

    Images

    • CDC2L5 antibody (ab58309) used in immunofluorescence at 10ug/ml on HeLa cells.


    • Predicted band size : 165 kDa
      CDC2L5 antibody (ab58309) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References

    ab58309 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Treatment
    siRNA 48hrs
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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