Preliminary experiments gave bands at approx 45kDa and 20kDa in Rat Eye lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 56.6kDa according to Rat NP_001101104.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Predicted to work with:
Mouse, Rat, Rabbit, Cow, Dog, Human, Chimpanzee, Monkey, Opossum
, corresponding to C terminal amino acids 492-501 of Human CDC2L6 (NP_055891.1)