Overview

  • Product nameAnti-Cdc37 antibody
    See all Cdc37 primary antibodies
  • Description
    Rabbit polyclonal to Cdc37
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1 -50 of Human Cdc37 (Q16543).

  • Positive control
    • Hela, 293T and mpuse NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCo-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
  • Sequence similaritiesBelongs to the CDC37 family.
  • Post-translational
    modifications
    Constitutively sumoylated by UBE2I.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 37 antibody
    • Cdc37 antibody
    • CDC37 cell division cycle 37 homolog antibody
    • CDC37 cell division cycle 37 S cerevisiae homolog antibody
    • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
    • Cdc37 homolog antibody
    • CDC37 protein antibody
    • CDC37_HUMAN antibody
    • CDC37A antibody
    • cell division cycle 37 antibody
    • Cell division cycle 37 homolog antibody
    • Hsp90 chaperone protein kinase targeting subunit antibody
    • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
    • Hsp90 chaperone protein kinase-targeting subunit antibody
    • Hsp90 co chaperone Cdc37 antibody
    • Hsp90 co-chaperone Cdc37 antibody
    • p50 antibody
    • p50Cdc37 antibody
    • S cerevisiae hypothetical protein CDC37 antibody
    see all

Anti-Cdc37 antibody images

  • All lanes : Anti-Cdc37 antibody (ab115166) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg


    Predicted band size : 44 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation of Cdc37 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdc37 antibody (ab115166); Lane 2-Immunoprecipitation with an anti-Cdc37 antibody that recognizes an downstream epitope ; Lane 3- Immunoprecipitation with IgG control. Anti-Cdc37 antibody (ab115166) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

References for Anti-Cdc37 antibody (ab115166)

ab115166 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (PC3 Prostate cancer cells)
Specification PC3 Prostate cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"