Anti-Cdc37 antibody (ab223361)
Key features and details
- Rabbit polyclonal to Cdc37
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc37 antibody
See all Cdc37 primary antibodies -
Description
Rabbit polyclonal to Cdc37 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Cdc37.
Database link: Q16543 -
Positive control
- WB: HeLa cell lysate. ICC/IF: Heat shocked HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200.
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WB |
1/2000. Predicted molecular weight: 44.5 kDa.
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Notes |
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ICC/IF
1/200. |
WB
1/2000. Predicted molecular weight: 44.5 kDa. |
Target
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Function
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
Sequence similarities
Belongs to the CDC37 family. -
Post-translational
modificationsConstitutively sumoylated by UBE2I. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11140 Human
- Omim: 605065 Human
- SwissProt: Q16543 Human
- Unigene: 160958 Human
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Alternative names
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
Images
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Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cdc37 (yellow) using ab223361 at 1/200 dilution in ICC/IF. Secondary antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200. The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.
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Anti-Cdc37 antibody (ab223361) at 1/2000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Developed using the ECL technique.
Predicted band size: 44.5 kDa -
2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cdc37 (green) using ab223361 at 1/200 dilution in ICC/IF. Secondary antibody: FITC Goat Anti-Rabbit at 1/200. The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223361 has not yet been referenced specifically in any publications.