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Rabbit polyclonal to Cdc37
ab61773 detects endogenous levels of total Cdc37 protein.
Synthesized non-phosphopeptide derived from human CDc37 around the phosphorylation site of serine 13 (E-V-SP-D-D).
- Human placenta tissue; extracts from NIH/3T3 cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. PubMed: 21163940
||1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
||1/50 - 1/100.
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
Belongs to the CDC37 family.
Constitutively sumoylated by UBE2I.
Information by UniProt
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
Anti-Cdc37 antibody images
Western blot - Cdc37 antibody (ab61773)
All lanes : Anti-Cdc37 antibody (ab61773) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells with no peptide
Lane 2 : extracts from NIH/3T3 cells with peptide
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 34 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc37 antibody (ab61773)
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61773 at a dilution of 1/50. Left: untreated sample. Right: sample treated with immunising peptide.
Western blot - Anti-Cdc37 antibody (ab61773)
All lanes :
Anti-Cdc37 antibody (ab61773) at 1/1000 dilutionLane 1 : Recombinant Human Cdc37 protein (ab78881) at 0.1 µg
Lane 2 : Recombinant Human Cdc37 protein (ab78881) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"