Overview

  • Product nameAnti-Cdc37 antibody
    See all Cdc37 primary antibodies
  • Description
    Rabbit polyclonal to Cdc37
  • Specificityab61773 detects endogenous levels of total Cdc37 protein.
  • Tested applicationsSuitable for: IP, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human CDc37 around the phosphorylation site of serine 13 (E-V-SP-D-D).

  • Positive control
    • Human placenta tissue; extracts from NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21163940
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • FunctionCo-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
  • Sequence similaritiesBelongs to the CDC37 family.
  • Post-translational
    modifications
    Constitutively sumoylated by UBE2I.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 37 antibody
    • Cdc37 antibody
    • CDC37 cell division cycle 37 homolog antibody
    • CDC37 cell division cycle 37 S cerevisiae homolog antibody
    • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
    • Cdc37 homolog antibody
    • CDC37 protein antibody
    • CDC37_HUMAN antibody
    • CDC37A antibody
    • cell division cycle 37 antibody
    • Cell division cycle 37 homolog antibody
    • Hsp90 chaperone protein kinase targeting subunit antibody
    • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
    • Hsp90 chaperone protein kinase-targeting subunit antibody
    • Hsp90 co chaperone Cdc37 antibody
    • Hsp90 co-chaperone Cdc37 antibody
    • p50 antibody
    • p50Cdc37 antibody
    • S cerevisiae hypothetical protein CDC37 antibody
    see all

Anti-Cdc37 antibody images

  • All lanes : Anti-Cdc37 antibody (ab61773) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells with no peptide
    Lane 2 : extracts from NIH/3T3 cells with peptide


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 34 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61773 at a dilution of 1/50. Left: untreated sample. Right: sample treated with immunising peptide.
  • All lanes : Anti-Cdc37 antibody (ab61773) at 1/1000 dilution

    Lane 1 : Recombinant Human Cdc37 protein (ab78881) at 0.1 µg
    Lane 2 : Recombinant Human Cdc37 protein (ab78881) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Anti-Cdc37 antibody (ab61773)

This product has been referenced in:
  • Soler-López M  et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, IP ; Human . Read more (PubMed: 21163940) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"